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Immobilization of Industrail Microbiology
It is technique used for the physical or chemical fixation of plant, animal cells, organelles, enzymes or other proteins (monoclonal antibodies) onto a solid matrix or retained by a membrane, in order to increase their stability and make possible their repeated or continued use.
The immobilized enzyme is defined as the enzyme physically confined or localized in a certain defined region of space with retention of its catalytic activity which can be used repeatedly and continuously.
The selection of appropriate carrier and immobilization procedure is very essential procedure is very essential for the immobilization technique.
Various types of materials like cellulose, dextran, agarose, gelatin, albumin polystyrene, Calcium alginate polyacrylamide, collagen carrageenan and polyurethane, inorganic materials (brick, rand, glass, and ceramics, magnetic) are used for immobilization.
The linkage is mediated by ionic bonds, physical absorption or bio specific binding.
The immobilization methods can be classified into four categories:-
- Carrier–binding
- Cross–linking
- Entrapping
- Combining
Among all these methods entrapping is discussed in brief.
Entrapping
The enzymes, cells are not directly attached to the support surface, but simply trapped inside the polymer matrix. Entrapping is carried out by mixing the biocatalyst into a monomer solution followed by a polymerization. It is done by change in temperature or by chemical reactions.
Advantages of immobilization
- Immobilized growing cells serve as self proliferating and self regenerating bio catalyst
- They are stable
- They are used either repeatedly in a series of batch wise reactions or continuously in flow systems.